Journal: npj Viruses

Article Title: Characterisation of a Japanese Encephalitis virus genotype 4 isolate from the 2022 Australian outbreak

doi: 10.1038/s44298-024-00025-5

Figure Lengend Snippet: A Photograph of “mini-brains” cultured in a rotating CelVivo Clinostar incubator. B IHC of viral antigen (4G4) for hBOs at 4 dpi. Images are representative of n = 4 hBOs for each group. Magnified images of sparse IMOJEV and YFV 17D infected cells are shown in Supplementary Fig. . C Viral growth kinetics up to 4 dpi determined by CCID 50 assays of culture supernatants at the indicated hours post infection; limit of detection is 2 log 10 CCID 50 /ml. At all time points JEV Nakayama vs. IMOJEV, and at 96 h JEV Nakayama vs. JEV NSW/22 were significant ( t tests, n = 5 organoids per group). D Mean percentage change in organoid area at 11 dpi vs. 9 dpi for each organoid ( n = 8 for uninfected and MVEV TC123130 , otherwise n = 4). Statistics are by Kolmogorov–Smirnov exact test for uninfected versus JEV Nakayama , and t test for uninfected versus JEV FU or JEV NSW/22 .

Article Snippet: A Photograph of “mini-brains” cultured in a rotating CelVivo Clinostar incubator.

Techniques: Cell Culture, Infection